6QAJ

Structure of the tripartite motif of KAP1/TRIM28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.28189
Spacegroup name	C 2 2 21
Unit cell lengths	59.774, 169.332, 374.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.798 - 2.901
R-factor	0.2625
Rwork	0.261
R-free	0.29100
Structure solution method	SAD
Starting model (for MR)	1lyd 2yvr
Data reduction software	XDS (Jan 26, 2018 BUILT=20180409)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHENIX (1.14-3260)
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	187.000	3.170
High resolution limit [Å]	2.630	2.630
Rmerge	0.078	0.832
Rpim	0.029	0.363
Number of reflections	33980	5596
<I/σ(I)>	13.4
Completeness [%]	32.4	3.9
Redundancy	8.5	6.1
CC(1/2)	0.998	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	15% (w/v) PEG 3350, 75 mM MgCl2, 0.1 M HEPES pH 7.5