6Q8I
Nterminal domain of human SMU1 in complex with human REDmid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 127.983, 68.163, 145.296 |
| Unit cell angles | 90.00, 109.40, 90.00 |
Refinement procedure
| Resolution | 49.000 - 3.170 |
| R-factor | 0.257 |
| Rwork | 0.256 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q8f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.250 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmeas | 0.144 | 1.146 |
| Number of reflections | 39441 | 2828 |
| <I/σ(I)> | 6.9 | 1.14 |
| Completeness [%] | 94.3 | 97.2 |
| Redundancy | 2.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Hepes pH 7.0-7.5, 8-10 % PEG 8K |
