6Q5Y

Crystal structure of the SPOC domain of human PHF3 in complex with RNA polymerase II CTD diheptapeptide phosphorylated on Ser2Ser5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0723
Spacegroup name	P 32
Unit cell lengths	67.832, 67.832, 178.118
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.760 - 2.850
R-factor	0.19394
Rwork	0.192
R-free	0.22689
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q2v
RMSD bond length	0.005
RMSD bond angle	1.385
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.530	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.157	1.432
Rmeas	0.183	1.662
Rpim	0.124	0.819
Number of reflections	21184	3104
<I/σ(I)>	6.6
Completeness [%]	98.8	99.5
Redundancy	3.5	3.6
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.03 M MgCl2, 0.03 M CaCl2, 0.1 M imidazole-MES pH 6.5, 20% glycerol and 10% PEG4000