6Q5A

Crystal structure of Cryptosporidium hominis CPSF3 in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 65 2 2
Unit cell lengths	90.360, 90.360, 238.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.490 - 1.550
R-factor	0.13969
Rwork	0.138
R-free	0.17931
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q55
RMSD bond length	0.008
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.660
High resolution limit [Å]	1.550	1.550
Rmeas	0.070	0.900
Number of reflections	82588	14867
<I/σ(I)>	15.7	2.6
Completeness [%]	98.0	96.8
Redundancy	10.1	9.3
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	273	17% (v/v) Poly Ethylene Glycol 4000, 10% (v/v) Isopropanol, 100 mM HEPES pH 7.5