6Q4M

Crystal structure of the O-GlcNAc transferase Asn648Tyr mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.873
Spacegroup name	F 2 2 2
Unit cell lengths	137.316, 150.736, 199.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.920 - 2.200
R-factor	0.17589
Rwork	0.175
R-free	0.19886
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.790
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.920	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	52373
<I/σ(I)>	12
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.45 M K2HPO4, 8 mM EDTA and 1% xylitol.