6Q45
F1-ATPase from Fusobacterium nucleatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.940, 200.209, 201.725 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 49.340 - 3.600 |
| R-factor | 0.2395 |
| Rwork | 0.237 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ik2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.759 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.290 | 49.290 | 3.660 |
| High resolution limit [Å] | 3.600 | 19.730 | 3.600 |
| Rmerge | 0.209 | 0.109 | 1.055 |
| Rmeas | 0.229 | 0.117 | 1.200 |
| Rpim | 0.090 | 0.043 | 0.555 |
| Total number of observations | 592580 | 3583 | 20356 |
| Number of reflections | 97166 | 570 | 4646 |
| <I/σ(I)> | 6.4 | 15.5 | 1.5 |
| Completeness [%] | 97.0 | 88.6 | 94.6 |
| Redundancy | 6.1 | 6.3 | 4.4 |
| CC(1/2) | 0.983 | 0.983 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1 ul protein to 0.8ul 100 mM sodium citrate, pH 6.0, 100 mM magnesium acetate and 15.5% [w/v] polyethylene glycol 5000 monomethyl ether and 0.2 ul low melting point agarose |
