6Q2X

TEAD4 (216-434) COMPLEXED WITH YAP PEPTIDE (60-100) AND MYRISTOATE (COVALENTLY BOUND) AT 2.1A (P41212 CRYSTAL FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	59.096, 59.096, 158.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.550 - 2.100
R-factor	0.233
Rwork	0.232
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kys
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.550	2.154
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.697
Rmeas	0.084	0.727
Rpim	0.084	0.471
Number of reflections	17209
<I/σ(I)>	22.9	4.2
Completeness [%]	99.8	100
Redundancy	12.6	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	16% PEG 3350,2% tacsimate, 0.1M NaCit