6Q2D

Crystal structure of Methanobrevibacter smithii Dph2 in complex with Methanobrevibacter smithii elongation factor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	100.899, 322.979, 172.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.356 - 3.450
R-factor	0.226
Rwork	0.224
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Methanobrevibacter smithii Dph2 Pyrococcus horikoshii EF-2 (5H7J)
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3211)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	3.640
High resolution limit [Å]	3.450	3.450
Rmerge	0.075
Number of reflections	37395	5369
<I/σ(I)>	8.7
Completeness [%]	99.7
Redundancy	4.1
CC(1/2)	0.998	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	The reservoir solution contained 100 mM Tris, pH 7.2, 200 mM MgSO4, and 21 % (w/v) PEG400; NaCl was added to the reservoir to a final concentration of 300 mM after forming the drop