6PZQ

Structure of the human respiratory syncytial virus M2-1 protein in complex with a short positive-sense gene-end RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-15
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	94.750, 94.750, 199.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.517 - 2.703
R-factor	0.2281
Rwork	0.225
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c3b
RMSD bond length	0.017
RMSD bond angle	1.470
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.750	2.850
High resolution limit [Å]	2.703	2.703
Rmerge	0.127
Rmeas	0.132
Rpim	0.027	0.390
Number of reflections	25701	3673
<I/σ(I)>	13.3
Completeness [%]	99.8	100
Redundancy	24.3	26
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 25% w/v polyethylene glycol 3,350