6PZH

Crystal structure of human NA-22 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	72.269, 74.542, 178.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.800 - 2.300
R-factor	0.1952
Rwork	0.193
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c2b 4v1d
RMSD bond length	0.005
RMSD bond angle	0.787
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.040	0.250
Number of reflections	43271	2184
<I/σ(I)>	23.4	2.2
Completeness [%]	99.3
Redundancy	4.7
CC(1/2)	0.996	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris, pH 8.5, 40% ethanol, 0.02% magnium chloride