6PZ3

Polymerase Eta-catalyzed insertion of correct G opposite template cytarabine (AraC) residue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-24
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	1.100
Spacegroup name	P 61
Unit cell lengths	98.221, 98.221, 81.348
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.531 - 2.395
R-factor	0.1761
Rwork	0.174
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	0.828
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	2.440
High resolution limit [Å]	2.395	6.510	2.400
Rmerge	0.132	0.045	0.910
Number of reflections	17653	922	865
<I/σ(I)>	6.4
Completeness [%]	100.0	100	100
Redundancy	19.1	18.8	19.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	MES BUFFER, PEG1500