6PYP
Binary Complex of Human Glycerol 3-Phosphate Dehydrogenase, R269A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.330, 76.560, 64.610 |
Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
Resolution | 42.000 - 1.950 |
Rwork | 0.167 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Starting model was an in-house model of open conformation |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.074 | 0.213 |
Rmeas | 0.103 | 0.295 |
Rpim | 0.071 | 0.202 |
Number of reflections | 44671 | 3113 |
<I/σ(I)> | 8.6 | 4.5 |
Completeness [%] | 99.7 | |
Redundancy | 3.1 | |
CC(1/2) | 0.993 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 23% PEG 20000 175 mM KCl 0.1M Bis-Tris propane |