6PY8

Crystal structure of the RBPJ-NOTCH1-NRARP ternary complex bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	79.880, 103.650, 301.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.460 - 3.750
R-factor	0.27
Rwork	0.267
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f8x
RMSD bond length	0.002
RMSD bond angle	0.573
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	3.888
High resolution limit [Å]	3.754	3.754
Number of reflections	26271	2504
<I/σ(I)>	7.39
Completeness [%]	99.2
Redundancy	4.4
CC(1/2)	0.998	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	50 mM Hepes pH 6.8, 200 mM Sodium Fluoride, 18% PEG 3350