6PXL

3.74 Angstroms resolution structure of HlsU with an axial-channel plug

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	414.594, 92.331, 200.849
Unit cell angles	90.00, 114.37, 90.00

Refinement procedure

Resolution	49.560 - 3.741
R-factor	0.2225
Rwork	0.220
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1do0
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3211)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.810
High resolution limit [Å]	3.740	3.740
Rmerge	0.121	0.562
Rmeas	0.140	0.694
Rpim	0.068	0.397
Number of reflections	68296	68296
<I/σ(I)>	10.3	1.17
Completeness [%]	94.6
Redundancy	3.8
CC(1/2)		0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonium sulfate, 18% PEG-3350, 0.1 M TRIS pH 7.5