6PPV

Structure of S. pombe Lsm1-7 with RNA, polyuridine with 3' guanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	69.023, 69.023, 296.068
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.070 - 2.050
R-factor	0.21
Rwork	0.209
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.700	2.110
High resolution limit [Å]	2.050	2.050
Number of reflections	52874	4047
<I/σ(I)>	11.6
Completeness [%]	100.0
Redundancy	21.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	20 mM sodium formate, 20 mM ammonium acetate, 20 mM trisodium citrate, 20 mM sodium potassium tartrate, 20 mM sodium oxamate, 100 mM sodium HEPES base, 100 mM MOPS acid, 10 % PEG 8,000, 20 % ethylene glycol