6POG

Crystal structure of the NELL2 EGF1-6-Robo3 FN1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-06-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	41.873, 90.439, 171.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.338 - 2.755
R-factor	0.2388
Rwork	0.237
R-free	0.26370
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	XDS (20170601)
Data scaling software	XDS (20170601)
Phasing software	AutoSol
Refinement software	PHENIX ((dev_3374: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.920
High resolution limit [Å]	2.755	2.755
Rmerge	0.106	1.599
Rmeas	0.116	1.736
Number of reflections	17564	2703
<I/σ(I)>	10.53	0.81
Completeness [%]	99.4	96.9
Redundancy	6.4	6.5
CC(1/2)	0.998	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	17% PEG 3,350, 0.4 M NaSCN