6PKY

Guinea pig N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain auto-inhibited by pro-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91977
Spacegroup name	P 41
Unit cell lengths	72.622, 72.622, 240.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.231 - 3.000
R-factor	0.2188
Rwork	0.212
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pkg
RMSD bond length	0.002
RMSD bond angle	0.604
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	24805	2460
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	23.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	295	20% PEG 3350, 0.1M bis-tris propane pH 8.25, 0.2M NaBr