6PKU
Guinea pig N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain (C51S C221S) in complex with N-acetyl-alpha-D-glucosamine (alpha-GlcNAc) and mannose 6-phosphate (M6P)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.459, 232.556, 65.305 |
| Unit cell angles | 90.00, 109.15, 90.00 |
Refinement procedure
| Resolution | 30.779 - 1.949 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pkg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.949 | 1.949 |
| Number of reflections | 149942 | 8692 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 10 mM tri-sodium citrate, 16% PEG 6000, soaked in 50 mM N-acetylglucosamine and 50 mM mannose 6-phosphate |
