6PKU

Guinea pig N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain (C51S C221S) in complex with N-acetyl-alpha-D-glucosamine (alpha-GlcNAc) and mannose 6-phosphate (M6P)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)	polymer	300	32493.1	4	UniProt (H0VTT5) Pfam (PF09992) In PDB	Cavia porcellus (Guinea pig)
2	A, B, C, D	2-acetamido-2-deoxy-alpha-D-glucopyranose	non-polymer		221.2	4	Chemie (NDG)
3	A, B, C, D	6-O-phosphono-alpha-D-mannopyranose	non-polymer		260.1	4	Chemie (M6P)
4	B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5		water	water		18.0	657	Chemie (HOH)

Sequence modifications

A, B, C, D: 39 - 327 (UniProt: H0VTT5)

PDB	External Database	Details
Asp 28	-	expression tag
Arg 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
Gly 36	-	expression tag
Ser 37	-	expression tag
Gly 38	-	expression tag
Ser 51	Cys 67	engineered mutation
Ser 221	Cys 237	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	129972.5
Non-Polymers*	Number of molecules	9
	Total formula weight	2146.6
All*	Total formula weight	132119.1

*Water molecules are not included.