6PGW

Crystal structure of zebrafish Protocadherin-19 EC3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	69.920, 58.896, 70.956
Unit cell angles	90.00, 117.97, 90.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.21939
Rwork	0.215
R-free	0.29815
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iu9
RMSD bond length	0.015
RMSD bond angle	1.474
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.078	0.229
Rmeas	0.102	0.304
Rpim	0.066	0.198
Number of reflections	10543	411
<I/σ(I)>	9.2	3.2
Completeness [%]	86.2	80.3
Redundancy	2	2
CC(1/2)		0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	277	100 mM HEPES, 55% MPD