6PGA

WDR5delta32 bound to methyl (4-(4-(hydroxymethyl)-1H-imidazol-2-yl)butyl)carbamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	46.782, 64.991, 97.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.170 - 2.450
R-factor	0.19942
Rwork	0.197
R-free	0.25028
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pg4
RMSD bond length	0.007
RMSD bond angle	1.390
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.170	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	11445	1242
<I/σ(I)>	5.9	2
Completeness [%]	99.9	99.4
Redundancy	12.4	12.8
CC(1/2)	0.980	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.237 M ammonium sulfate, 0.1 M bis-tris chloride, 25.7 %w/v MPEG5000