6PFN
Succinyl-CoA synthase from Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-13 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.073, 158.735, 73.017 |
| Unit cell angles | 90.00, 113.11, 90.00 |
Refinement procedure
| Resolution | 49.650 - 1.760 |
| R-factor | 0.1873 |
| Rwork | 0.186 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mgg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.650 | 49.650 | 1.790 |
| High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
| Rmerge | 0.074 | 0.040 | 0.508 |
| Rmeas | 0.088 | 0.047 | 0.646 |
| Rpim | 0.047 | 0.025 | 0.391 |
| Number of reflections | 138572 | 7083 | 6079 |
| <I/σ(I)> | 6.6 | 1.48 | |
| Completeness [%] | 97.9 | 98.5 | 86.9 |
| Redundancy | 3.3 | 3.4 | 2.5 |
| CC(1/2) | 0.997 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M ammonium acetate, 0.1 M bis-tris 5.5, 17% polyethylene glycol 10,000 |
