6PD5
Crystal Structure of a H5N1 influenza virus hemagglutinin at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 108.056, 108.056, 419.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.350 - 2.390 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fk0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.520 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmeas | 0.163 | 1.764 |
| Number of reflections | 37825 | 5929 |
| <I/σ(I)> | 12.29 | 2.57 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 9.14 | 8.65 |
| CC(1/2) | 0.997 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 mM cacodylate, pH 6.5, 200 mM sodium chloride, 2 M ammonium sulfate, 10% glycerol |
