6PBK
Archaellum periplasmic stator protein complex FlaF and FlaG from Sulfolobus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 |
| Unit cell lengths | 120.038, 120.038, 152.138 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.292 - 2.805 |
| R-factor | 0.1728 |
| Rwork | 0.171 |
| R-free | 0.20210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p94 5tuh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.190 | 49.190 | 2.960 |
| High resolution limit [Å] | 2.800 | 8.870 | 2.800 |
| Rmerge | 0.083 | 0.025 | 1.265 |
| Rmeas | 0.090 | 0.027 | 1.370 |
| Rpim | 0.034 | 0.010 | 0.521 |
| Total number of observations | 206623 | 6445 | 30017 |
| Number of reflections | 30449 | 992 | 4409 |
| <I/σ(I)> | 16.4 | 56.3 | 1.5 |
| Completeness [%] | 99.9 | 99.2 | 99.7 |
| Redundancy | 6.8 | 6.5 | 6.8 |
| CC(1/2) | 0.999 | 1.000 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 18% PEG 6000, 0.1 M Tris, pH 7.5, and 0.2 M NaBr |
