6PBC

Structural basis for the activation of PLC-gamma isozymes by phosphorylation and cancer-associated mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	70.766, 82.441, 228.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.220 - 2.460
R-factor	0.2049
Rwork	0.202
R-free	0.24470
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.586
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.500
High resolution limit [Å]	2.460	6.670	2.460
Rmerge	0.123	0.038	0.691
Rmeas	0.132	0.041	0.748
Rpim	0.046	0.014	0.281
Total number of observations	400272
Number of reflections	49513	2718	2257
<I/σ(I)>	6.8
Completeness [%]	99.6	99.6	92.3
Redundancy	8.1	7.5	6.8
CC(1/2)		0.999	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12.5% w/v PEG 3,350, 50 mM di-sodium tartrate, 5% v/v glycerol