6P96
OXA-48 carbapanemase, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.746, 76.827, 100.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.413 - 1.600 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.413 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 72537 | 3522 |
| <I/σ(I)> | 13.8 | 1.7 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 13.1 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5 mM Ca2+, 5 mM Co2+, 5 mM Cd2+, 20% PEG 3350 |
