6P96

OXA-48 carbapanemase, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	70.746, 76.827, 100.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.413 - 1.600
R-factor	0.185
Rwork	0.184
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.413	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	72537	3522
<I/σ(I)>	13.8	1.7
Completeness [%]	99.9	99.2
Redundancy	13.1	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5 mM Ca2+, 5 mM Co2+, 5 mM Cd2+, 20% PEG 3350