6P91

Structure of Lassa virus glycoprotein bound to Fab 18.5C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9796
Spacegroup name	I 21 3
Unit cell lengths	231.660, 231.660, 231.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.610 - 4.000
R-factor	0.2065
Rwork	0.204
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vk2 4fql
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.370	49.370	4.150
High resolution limit [Å]	3.790	9.290	3.790
Rmerge	0.201	0.060	4.502
Rmeas	0.207	0.062	4.625
Rpim	0.047	0.015	1.048
Total number of observations		25298	92817
Number of reflections	20596	1466	4861
<I/σ(I)>	11	41.5	0.9
Completeness [%]	99.8	99.4	99.5
Redundancy	19.8	17.3	19.1
CC(1/2)	0.999	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.15-0.2 M ammonium sulfate, 0.1M Tris pH 5-8.5