6P8E

Crystal structure of CDK4 in complex with CyclinD1 and P27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	62.410, 67.490, 187.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.640 - 2.300
R-factor	0.1751
Rwork	0.172
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.886
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.640	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.130	1.080
Number of reflections	35354	4854
<I/σ(I)>	9.9	2.2
Completeness [%]	98.2	94.5
Redundancy	7.8	7.2
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	100mM Tris (7.0) 200mM MgCl2 25%PEG 8000