6P7E
Structure of T7 DNA Polymerase Bound to a Primer/Template DNA and a Peptide that Mimics the C-terminal Tail of the Primase-Helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-01 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 |
| Unit cell lengths | 100.724, 102.698, 148.885 |
| Unit cell angles | 91.36, 96.83, 113.11 |
Refinement procedure
| Resolution | 49.129 - 3.001 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ajq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.129 | 3.030 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 107350 | 3336 |
| <I/σ(I)> | 7.64 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | 25% PEG 4000, 0.1M LiSO4, and 0.1 M Tris pH 7.5 |
