6P74

OLD nuclease from Thermus Scotoductus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0718
Spacegroup name	I 21 21 21
Unit cell lengths	83.362, 101.737, 202.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	101.377 - 2.200
R-factor	0.1943
Rwork	0.193
R-free	0.22910
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.380	2.180
High resolution limit [Å]	2.120	2.120
Number of reflections	49235	741
<I/σ(I)>	17.6
Completeness [%]	100.0
Redundancy	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M NaOAc pH 5.8, 0.3 M AmSO4, 7% PEG MME 2000, and 5 mM SmCl3