6P70
X-ray crystal structure of bacterial RNA polymerase and pyrBI promoter complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.179, 100.845, 294.865 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 46.061 - 3.052 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vo8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.451 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.130 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rpim | 0.068 | 0.549 |
| Number of reflections | 101835 | 4930 |
| <I/σ(I)> | 10.9 | 1.21 |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 3.4 | 2.9 |
| CC(1/2) | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 9.5 % PEG4000 |
