6P66
The crystal structure of the XPB complex with Bax1 from Archaeoglobus fulgidus at 3.0 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.600, 129.840, 108.150 |
| Unit cell angles | 90.00, 104.30, 90.00 |
Refinement procedure
| Resolution | 39.932 - 3.000 |
| R-factor | 0.2266 |
| Rwork | 0.225 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fwr |
| Data reduction software | MOSFLM (7.2.0) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 107.969 | 39.932 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.034 | 0.670 | |
| Rmeas | 0.090 | 0.042 | 0.908 |
| Rpim | 0.048 | 0.022 | 0.472 |
| Total number of observations | 182895 | 5885 | 27385 |
| Number of reflections | 52884 | 1650 | 7664 |
| <I/σ(I)> | 8.4 | 29.8 | 1.4 |
| Completeness [%] | 98.5 | 94.1 | 98.4 |
| Redundancy | 3.5 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1:1 ratio of protein to mother liquor (0.1 M sodium acetate pH 4.8, 1.8 M sodium acetate) |
