6P59

Crystal structure of SIVrcm Vif-CBFbeta-ELOB-ELOC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11583
Spacegroup name	P 21 21 21
Unit cell lengths	110.220, 112.489, 125.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.117 - 2.942
R-factor	0.236271019504
Rwork	0.235
R-free	0.26846
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n9f
RMSD bond length	0.006
RMSD bond angle	0.862
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	3.047
High resolution limit [Å]	2.940	2.940
Number of reflections	33445	3146
<I/σ(I)>	12.27
Completeness [%]	98.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MES (pH 6), PEG 6000, Dichloromethane