6P4W
XPB helicase in a complex with truncated Bax1 from Sulfurisphaera tokodaii at 2.96 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.415, 101.369, 114.476 |
| Unit cell angles | 83.09, 81.15, 90.17 |
Refinement procedure
| Resolution | 29.280 - 2.956 |
| R-factor | 0.1855 |
| Rwork | 0.183 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tnu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
| Rmerge | 0.098 | 0.053 | 0.822 |
| Rmeas | 0.116 | 0.062 | 0.982 |
| Rpim | 0.061 | 0.033 | 0.529 |
| Total number of observations | 179503 | ||
| Number of reflections | 51223 | 2569 | 2523 |
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 99.1 | 99.7 | 98.3 |
| Redundancy | 3.5 | 3.5 | 3.3 |
| CC(1/2) | 0.996 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 200 mM Ammonium citrate, pH 7.5, 8% PEG 3350 |
