6P24
Escherichia coli tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.269, 173.583, 251.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.040 - 2.120 |
R-factor | 0.2299 |
Rwork | 0.229 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pco |
RMSD bond length | 0.002 |
RMSD bond angle | 0.458 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 173.580 | 2.150 |
High resolution limit [Å] | 2.115 | 2.115 |
Number of reflections | 152649 | 6642 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 98.7 | |
Redundancy | 3.3 | |
CC(1/2) | 0.994 | 0.126 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% PEG 8,000, 200 mM MgCl2, 100 mM Tris-HCl pH8.5, 2% 1,6-hexanediol, 3% d-Galactose |