6P1T

Pre-catalytic ternary complex of human DNA Polymerase Mu with 1-nt gapped substrate containing template 8OG and bound CMPCPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.805, 68.715, 110.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.042 - 1.700
R-factor	0.1702
Rwork	0.169
R-free	0.19090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.087
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmeas	0.056	0.633
Rpim	0.016	0.317
Number of reflections	49662	1927
<I/σ(I)>	31.6	2.09
Completeness [%]	97.6	77.5
Redundancy	8.5	3.2
CC(1/2)		0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	94mM HEPES pH 7.5, 94mM NaCl, 9.4% w/v PEG4000