6OYL

The structure of the PP2A B56 subunit KIF4A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.290, 108.037, 117.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.518 - 3.150
R-factor	0.2162
Rwork	0.215
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k6s
RMSD bond length	0.003
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.520	3.360
High resolution limit [Å]	3.148	3.148
Rmerge	0.105
Rmeas	0.113
Number of reflections	11932	1801
<I/σ(I)>	11.5
Completeness [%]	96.6	83.1
Redundancy	8.2	7.7
CC(1/2)		0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.75	298	0.1 M HEPES, pH 7.75, 0.8 M lithium chloride, 8% PEG8000