6OYL
The structure of the PP2A B56 subunit KIF4A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.290, 108.037, 117.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.518 - 3.150 |
| R-factor | 0.2162 |
| Rwork | 0.215 |
| R-free | 0.23120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k6s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.652 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.520 | 3.360 |
| High resolution limit [Å] | 3.148 | 3.148 |
| Rmerge | 0.105 | |
| Rmeas | 0.113 | |
| Number of reflections | 11932 | 1801 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 96.6 | 83.1 |
| Redundancy | 8.2 | 7.7 |
| CC(1/2) | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 0.1 M HEPES, pH 7.75, 0.8 M lithium chloride, 8% PEG8000 |
