6OX5

A SETD3 Mutant (N255A) in Complex with an Actin Peptide with His73 Replaced with Lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	60.465, 116.497, 174.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.938 - 2.098
R-factor	0.1649
Rwork	0.163
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mbj
RMSD bond length	0.004
RMSD bond angle	0.632
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.940	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.158	1.022
Rpim	0.036	0.450
Number of reflections	35098	2968
<I/σ(I)>	18.5	2.2
Completeness [%]	96.6	82.9
Redundancy	16.1	5.5
CC(1/2)		0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000