6OX2
SETD3in Complex with an Actin Peptide with the Target Histidine Fully Methylated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.098, 175.941, 66.264 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 43.560 - 2.089 |
| R-factor | 0.2002 |
| Rwork | 0.199 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mbj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.570 | 2.160 |
| High resolution limit [Å] | 2.089 | 2.089 |
| Rmerge | 0.142 | 0.911 |
| Rpim | 0.060 | 0.443 |
| Number of reflections | 78578 | 7372 |
| <I/σ(I)> | 12.6 | 1.6 |
| Completeness [%] | 97.0 | 91.5 |
| Redundancy | 6.1 | |
| CC(1/2) | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |
