6OS0

Structure of synthetic nanobody-stabilized angiotensin II type 1 receptor bound to angiotensin II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	135.530, 37.830, 111.920
Unit cell angles	90.00, 94.41, 90.00

Refinement procedure

Resolution	37.196 - 2.900
R-factor	0.2652
Rwork	0.259
R-free	0.31800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6do1
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.196	37.196	2.980
High resolution limit [Å]	2.900	12.970	2.900
Rmerge	0.276	0.097	0.806
Rmeas	0.341	0.125	1.002
Total number of observations	27811
Number of reflections	11842	142	858
<I/σ(I)>	2.25	5.13	0.61
Completeness [%]	91.2	82.1	93.1
Redundancy	2.349	2.261	2.373
CC(1/2)	0.926	0.990	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.6	293	Protein complex was reconstituted with a 10:1 (w/w) mixture of monoolein and cholesterol. Crystals were grown in 100 mM Tris pH 7.6, 10 mM MgCl2, 25-26% PEG 300