6OS0
Structure of synthetic nanobody-stabilized angiotensin II type 1 receptor bound to angiotensin II
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Type-1 angiotensin II receptor,Soluble cytochrome b562 BRIL fusion protein | polymer | 425 | 48514.9 | 1 | UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001) Pfam (PF07361) | Homo sapiens (Human) | AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1,Cytochrome b-562 |
| 2 | B (D) | Nanobody Nb.AT110i1 | polymer | 126 | 13712.0 | 1 | synthetic construct | ||
| 3 | C (B) | Angiotensinogen | polymer | 8 | 1048.2 | 1 | UniProt (P01019) | Homo sapiens | Serpin A8 |
| 4 | D (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 5 | E, F (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 6 | G, H (A, D) | water | water | 18.0 | 18 | Chemie (HOH) |
Sequence modifications
A: 2 - 226 (UniProt: P30556)
A: 227 - 1226 (UniProt: P0ABE7)
A: 1227 - 1319 (UniProt: P30556)
| PDB | External Database | Details |
|---|---|---|
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Lys 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Trp 232 | Met 29 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 63275.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 292.1 | |
| All* | Total formula weight | 63567.2 |
