6OQY

Human LRH-1 bound to the agonist 6N and a fragment of the Tif2 coregulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	73
Detector technology	PIXEL
Collection date	2018-03-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	46.683, 46.683, 217.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.274 - 2.230
R-factor	0.2322
Rwork	0.230
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l11
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.230	2.230
Number of reflections	12206	1055
<I/σ(I)>	8.9
Completeness [%]	96.8
Redundancy	16.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium acetate (pH 4.6), PEG4000, glycerol