6OQM
crystal structure of the MSH6 PWWP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 2 |
Unit cell lengths | 56.421, 56.421, 176.291 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.382 - 2.200 |
R-factor | 0.2217 |
Rwork | 0.217 |
R-free | 0.26980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vc8 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.090 | 47.090 | 2.270 |
High resolution limit [Å] | 2.200 | 9.060 | 2.200 |
Rmerge | 0.056 | 0.022 | 1.325 |
Rmeas | 0.059 | 0.024 | 1.392 |
Rpim | 0.018 | 0.008 | 0.423 |
Total number of observations | 61628 | ||
Number of reflections | 5806 | 106 | 497 |
<I/σ(I)> | 25.6 | ||
Completeness [%] | 99.9 | 99.3 | 98.8 |
Redundancy | 10.6 | 7.8 | 10.7 |
CC(1/2) | 1.000 | 0.999 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.6 M ammonium sulfate, 0.1 M sodium cacodylate pH 5.5, 0.01 M magnesium chloride |