6OOV
Crystal structure of HMCES SRAP domain in complex with palindromic 3' overhang DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.729, 139.828, 66.613 |
| Unit cell angles | 90.00, 99.99, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.200 |
| R-factor | 0.1948 |
| Rwork | 0.194 |
| R-free | 0.21920 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6oeb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.840 | 47.840 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.056 | 0.018 | 0.646 |
| Rmeas | 0.066 | 0.022 | 0.771 |
| Rpim | 0.035 | 0.012 | 0.416 |
| Total number of observations | 142073 | 2049 | 11999 |
| Number of reflections | 41672 | 619 | 3610 |
| <I/σ(I)> | 13.8 | 48.5 | 1.7 |
| Completeness [%] | 99.7 | 99.3 | 99.9 |
| Redundancy | 3.4 | 3.3 | 3.3 |
| CC(1/2) | 0.999 | 0.999 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 11% PEG 8K, 0.1M CaCl2, 0.1M Hepes |
