6OON
Human Argonaute4 bound to guide RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.980, 68.011, 83.270 |
| Unit cell angles | 90.00, 102.68, 90.00 |
Refinement procedure
| Resolution | 49.082 - 1.900 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.082 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 91128 | 14243 |
| <I/σ(I)> | 1.91 | |
| Completeness [%] | 98.9 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | PEG5000, 0.1 M MES, pH=6, 1 M LiCl, 100 mM Trimethylamine Hydrochloride |
