6OMS
Arabidopsis GH3.12 with Chorismate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.438, 67.002, 100.964 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 48.589 - 1.942 |
| R-factor | 0.1876 |
| Rwork | 0.186 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eql |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 85974 | 0 |
| <I/σ(I)> | 8.9 | 1.8 |
| Completeness [%] | 98.7 | 94.3 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% PEG-3350, 0.25 M ammonium acetate, 100 mM sodium acetate pH 4.4, 5 mM tris(2-carboxyethyl)phosphine, 5 mM MgCl2, 10 mM chorismate, and 4 mM AMP |
