6OIB
Crystal structure of human Sulfide Quinone Oxidoreductase in complex with coenzyme Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.516, 111.512, 133.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.370 - 2.030 |
| R-factor | 0.2099 |
| Rwork | 0.209 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.460 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.184 | 1.664 |
| Rpim | 0.079 | 0.798 |
| Number of reflections | 73848 | 4449 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 96.9 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M ammonium tartrate dibasic, 20% PEG 3350 |
