6OGK
MeCP2 MBD in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.470, 51.976, 66.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.900 - 1.650 |
| R-factor | 0.2289 |
| Rwork | 0.226 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c1y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.743 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.910 | 40.910 | 1.630 |
| High resolution limit [Å] | 1.600 | 8.780 | 1.600 |
| Rmerge | 0.060 | 0.014 | 1.835 |
| Rmeas | 0.065 | 0.016 | 2.092 |
| Rpim | 0.026 | 0.007 | 0.982 |
| Total number of observations | 115783 | 726 | 3650 |
| Number of reflections | 18969 | ||
| <I/σ(I)> | 17.5 | 82.5 | 0.7 |
| Completeness [%] | 99.8 | 99.5 | 97.3 |
| Redundancy | 6.1 | 4.8 | 4.1 |
| CC(1/2) | 1.000 | 1.000 | 0.366 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 50% precipitant mix 4, 0.1M buffer system 1, 0.06M divalents (morpheus A4) |
