6OGJ
MeCP2 MBD in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.723, 90.181, 40.524 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 45.090 - 1.800 |
| R-factor | 0.2278 |
| Rwork | 0.226 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c1y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.090 | 45.090 | 1.830 |
| High resolution limit [Å] | 1.800 | 8.990 | 1.800 |
| Rmerge | 0.047 | 0.021 | 1.111 |
| Rmeas | 0.056 | 0.025 | 1.336 |
| Rpim | 0.030 | 0.013 | 0.732 |
| Total number of observations | 65521 | 556 | 3431 |
| Number of reflections | 19585 | ||
| <I/σ(I)> | 12 | 40.1 | 0.9 |
| Completeness [%] | 98.6 | 99 | 94.1 |
| Redundancy | 3.3 | 3.3 | 3 |
| CC(1/2) | 0.999 | 0.999 | 0.406 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG 2000 MME, 0.2M potassium bromide |
