Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977410 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 55.863, 52.060, 148.309 |
| Unit cell angles | 90.00, 93.11, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.100 |
| R-factor | 0.2098 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ko9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.563 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.110 | 49.110 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.064 | 0.033 | 0.812 |
| Rmeas | 0.069 | 0.036 | 0.882 |
| Rpim | 0.027 | 0.014 | 0.341 |
| Total number of observations | 165977 | 2186 | 13611 |
| Number of reflections | 25066 | 360 | 2067 |
| <I/σ(I)> | 15 | 45.7 | 2.2 |
| Completeness [%] | 99.9 | 98.9 | 99.9 |
| Redundancy | 6.6 | 6.1 | 6.6 |
| CC(1/2) | 0.999 | 0.998 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 20% PEG3350, 0.1 M potassium chloride, 0.1 M Bis-Tris, 0.05 M magnesium chloride |
